Upstream / Downstream
Explore pathways related to this product.
Important Ordering DetailsCustom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at firstname.lastname@example.org.
CST Antibody Performance Guarantee
Find answers on our FAQs page.
PTM information and tools available.
SignalKine™ Human IL-4 Sandwich ELISA Kit #8669
This product is discontinued
Figure 1. PBMCs were initially stimulated with immobilized anti-CD3 (20 mg/well), soluble anti-CD28 antibody (1 μg/ml), recombinant hIL-4 (1 ng/ml), and recombinant hIL-2 (2 ng/ml) for a total of 5 days. The cells were harvested, washed, and treated with TPA #4174 (10 ng/ml) and ionomycin (1 μg/ml). Supernatants were removed after 4 and 24 hours and compared with supernatants from untreated cells using the SignalKine™ Human IL-4 Sandwich ELISA Kit.Learn more about how we got this image
Gallery: SignalKine™ Human IL-4 Sandwich ELISA Kit #8669
- Figure 1. PBMCs were initially stimulated with immobilized anti-CD3 (20 mg/well), soluble anti-CD28 antibody (1 μg/ml), recombinant hIL-4 (1 ng/ml), and recombinant hIL-2 (2 ng/ml) for a total of 5 days. The cells were harvested, washed, and treated with TPA #4174 (10 ng/ml) and ionomycin (1 μg/ml). Supernatants were removed after 4 and 24 hours and compared with supernatants from untreated cells using the SignalKine™ Human IL-4 Sandwich ELISA Kit.
- Sample Testing
|Product Includes||Volume||Solution Color|
|IL-4 Rabbit mAb Coated Microwells||96 tests|
|Human IL-4 Standard (Lyophilized)||1 vial|
|SignalKine™ Assay Diluent A01||1.5 ml||Colorless|
|SignalKine™ Sample Diluent S01||25 ml||Brown|
|IL-4 Detection Rabbit mAb (Lyophilized) 100X||1 vial|
|Detection Antibody Diluent||6 ml||Green|
|HRP-linked Streptavidin (Lyophilized) 100X||1 vial|
|HRP Diluent||6 ml||Red|
|ELISA Wash Buffer (20X)||25 ml||Colorless|
|TMB Substrate 7004||6 ml||Colorless|
|STOP Solution 7002||6 ml||Colorless|
|Sealing Tape||2 sheets|
SignalKine™ Human IL-4 Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human IL-4 (hIL-4) in multiple matrices. Unknown samples being tested for hIL-4 and hIL-4 standards are added to low volume microwells, where the hIL4 is captured by the coated hIL-4 Rabbit mAb. Following a washing step, a biotinylated hIL-4 Detection Rabbit mAb is added to detect the captured hIL-4. HRP-linked Streptavidin is then used for detection of the biotinylated hIL-4 Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of hIL-4 in the sample.
SignalKine™ Human IL-4 Sandwich ELISA Kit detects hIL-4 in multiple matrices that can be quantified by generating a standard curve with the recombinant hIL-4 protein standard provided. The hIL-4 standard range is from 15.6 to 1000 pg/ ml. Samples containing higher levels of hIL-4 can be diluted to fit into the standards range.
Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.