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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

IL-1β Antibody (Mouse Specific) recognizes endogenous levels of full length mouse IL-1β protein. This antibody can detect 500pg of mature recombinant mouse IL-1β by western blot.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IL-1β protein. Antibodies are purified by protein A and peptide affinity chromatography.

Interleukin-1β (IL-1β) is a proinflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP); the complex then binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII, which lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).

1.  Dinarello, C.A. (1996) Blood 87, 2095-147.

2.  Allan, S.M. et al. (2005) Nat Rev Immunol 5, 629-40.

3.  Kramer, F. et al. (2008) Mol Immunol 45, 2678-89.

Entrez-Gene Id 16176
Swiss-Prot Acc. P10749

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

IL-1β Antibody (Mouse Specific)