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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
G9a/EHMT2 (C6H3) Rabbit mAb 3306 1 x 40 µl
H M R Mk 140, 165 Rabbit IgG
ESET (C1C12) Rabbit mAb 2196 1 x 40 µl
H Mk 180 Rabbit IgG
ASH2L (D93F6) XP® Rabbit mAb 5019 1 x 40 µl
H M R Mk 80, 65 Rabbit IgG
RBBP5 (D3I6P) Rabbit mAb 13171 1 x 40 µl
H M R Mk 70 Rabbit IgG
SET7/SET9 Antibody 2813 1 x 40 µl
H M R Mk 48 Rabbit 
SMYD2 (D14H7) Rabbit mAb 9734 1 x 40 µl
H M R Mk 49 Rabbit IgG
SUV39H1 (D11B6) Rabbit mAb 8729 1 x 40 µl
H M R Mk 48 Rabbit IgG
SET8 (C18B7) Rabbit mAb 2996 1 x 40 µl
H M R Mk 43 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 1 x 100 µl
All Goat 

Product Description

The Lysine Methyltransferase Antibody Sampler Kit provides a fast and economical means to evaluate endogenous levels of lysine methyltransferases. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody in this kit recognizes only the specific target protein and does not cross-react with other family members.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ASH2L protein, the carboxy terminus of human ESET protein, the carboxy terminus of human G9a/EHMT2 protein, human SET8 protein, residues surrounding Val414 of human SMYD2 protein, residues surrounding Val408 of human RBBP5 protein, or residues surrounding Asp380 of human SUV39H1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues the human SET7/SET9 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

SET domain-containing proteins are potential histone methyltransferases (HMTases), which are classified into subgroups by their putative substrate specificities. Histone H3 Lys9 (H3-K9) methyltransferase group genes include Suv39h1, Suv39h2, G9a, G9a related protein (GLP) and SETDB1/ESET (1). The H3-K9 methylation mark plays an important role as a binding site for the chromo-containing protein, resulting in chromatin compaction and heterochromatin generation (2). Histone H3-K4 methylation is exclusively associated with actively transcribed genes (2). The first H3-K4 methylase complex, COMPASS, was identified in the yeast S. cerevisiae and consists of Set1/KMT2 and seven other polypeptides, Cps60-Cps15 (2). Set1/KMT2 functions within COMPASS and is capable of mono-, di-, and trimethylating H3-K4 (2). There are several Set1 related proteins in mammals including WDR5, RBBP5, ASH2L, CXXC1, and DPY30 (2,3). SET7/SET9 is a member of the SET domain-containing family that can specifically methylate H3-K4, Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (4-6). SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a single-subunit enzyme that mono-methylates histone H4-K20, preferably on nucleosomal substrates (7-9). SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), functions to repress transcription by interacting with the Sin3A repressor complex and methylating H3-K36 (10). SMYD2 also methylates H3-K4 through interaction with HSP90α, and methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (11,12).

1.  Tachibana, M. et al. (2005) Genes Dev 19, 815-26.

2.  Fang, J. et al. (2002) Curr Biol 12, 1086-99.

3.  Brown, M.A. et al. (2006) Mol Cancer 5, 26.

4.  Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.

5.  Xiao, B. et al. (2005) Genes Dev 19, 1444-54.

6.  Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72.

7.  Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.

8.  Huang, J. et al. (2006) Nature 444, 629-32.

9.  Nishioka, K. et al. (2002) Genes Dev 16, 479-89.

10.  Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.

11.  Kouskouti, A. et al. (2004) Mol Cell 14, 175-82.

12.  Chuikov, S. et al. (2004) Nature 432, 353-60.

Entrez-Gene Id 9070, 9869, 10919, 5929, 80854, 387893, 56950, 6839
Swiss-Prot Acc. Q9UBL3, Q15047, Q96KQ7, Q15291, Q8WTS6, Q9NQR1, Q9NRG4, O43463

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.