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REACTIVITY SENSITIVITY MW (kDa) SOURCE
86 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Stat3α Antibody recognizes endogenous levels of total Stat3α protein. This antibody does not cross-react with Stat3β.


Species predicted to react based on 100% sequence homology: Dog

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly738 of human Stat3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).


1.  Darnell, J.E. et al. (1994) Science 264, 1415-21.

2.  Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.

3.  Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.

4.  Bromberg, J.F. et al. (1999) Cell 98, 295-303.

5.  Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.

6.  Wen, Z. et al. (1995) Cell 82, 241-50.

7.  Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.

8.  Ihle, J.N. (1995) Nature 377, 591-4.

9.  Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

10.  Yokogami, K. et al. (2000) Curr Biol 10, 47-50.


Entrez-Gene Id 6774
Swiss-Prot Acc. P40763


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

8719
Stat3α Antibody