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REACTIVITY SENSITIVITY MW (kDa) SOURCE
25 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Atg101 Antibody recognizes endogenous levels of total Atg101 protein. This antibody also detects a 55 kDa protein of unknown origin in some cell lines.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val177 of human Atg101 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Atg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2).


1.  Jung CH et al. (2009) Mol Biol Cell 20, 1992–2003

2.  Mercer, C.A. et al. (2009) Autophagy 5, 649-62.

3.  Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.

4.  Hosokawa, N. et al. (2009) Autophagy 5, 973-9.

5.  Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.

6.  Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.

7.  Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.

8.  Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74.

9.  Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.


Entrez-Gene Id 60673
Swiss-Prot Acc. Q9BSB4


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