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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Spry1 Antibody recognizes endogenous levels of total Spry1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro144 of human Spry1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Spry1 is a member of the Sprouty (Spry) family proteins that was initially identified in Drosophila as an inhibitor of the FGF signaling pathway (1). There are four human Spry proteins (Spry1-4), encoded by different genes, and they all share a highly conserved carboxy-terminal cystine-rich Spry domain that is known to be essential for their receptor tyrosine kinase inhibitory function stimulated by various growth factors (1-3). Spry1 and other Spry proteins play a key role in embryonic development, tissue and organ formation, as well as growth in almost all living organisms (1-4). Spry proteins are considered tumor suppressors due to their inhibitory function in a variety of growth factor signaling pathways (2,3). Spry1 anchors itself to the membrane by palmitoylation and can translocate from the cytosol to the membrane by binding to caveolin-1 (5,6). Regulation of Spry1 protein function is thought to occur at various levels. Spry1 regulation includes transcriptional regulation by growth factors and kinases (1,4,7), post-transcriptional regulation by microRNA-21 (8), post-translational modifications including phosphorylation, dephosphorylation, ubiquitination and proteasomal degradation, and regulation by its interacting protein partners (2,3).

1.  Hacohen, N. et al. (1998) Cell 92, 253-63.

2.  Guy, G.R. et al. (2009) J Endocrinol 203, 191-202.

3.  Minowada, G. et al. (1999) Development 126, 4465-75.

4.  Impagnatiello, M.A. et al. (2001) J Cell Biol 152, 1087-98.

5.  Hanafusa, H. et al. (2002) Nat Cell Biol 4, 850-8.

6.  Ozaki, K. et al. (2001) Biochem Biophys Res Commun 285, 1084-8.

7.  Thum, T. et al. (2008) Nature 456, 980-4.

8.  Edwin, F. et al. (2009) Mol Pharmacol 76, 679-91.

Entrez-Gene Id 10252
Swiss-Prot Acc. O43609

For Research Use Only. Not For Use In Diagnostic Procedures.
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Spry1 Antibody