Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

We recommend the following alternatives

W IP         H M
W IP         H M R
REACTIVITY SENSITIVITY MW (kDa) SOURCE
35 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Spry1 Antibody recognizes endogenous levels of total Spry1 protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro144 of human Spry1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Spry1 is a member of the Sprouty (Spry) family proteins that was initially identified in Drosophila as an inhibitor of the FGF signaling pathway (1). There are four human Spry proteins (Spry1-4), encoded by different genes, and they all share a highly conserved carboxy-terminal cystine-rich Spry domain that is known to be essential for their receptor tyrosine kinase inhibitory function stimulated by various growth factors (1-3). Spry1 and other Spry proteins play a key role in embryonic development, tissue and organ formation, as well as growth in almost all living organisms (1-4). Spry proteins are considered tumor suppressors due to their inhibitory function in a variety of growth factor signaling pathways (2,3). Spry1 anchors itself to the membrane by palmitoylation and can translocate from the cytosol to the membrane by binding to caveolin-1 (5,6). Regulation of Spry1 protein function is thought to occur at various levels. Spry1 regulation includes transcriptional regulation by growth factors and kinases (1,4,7), post-transcriptional regulation by microRNA-21 (8), post-translational modifications including phosphorylation, dephosphorylation, ubiquitination and proteasomal degradation, and regulation by its interacting protein partners (2,3).


1.  Hacohen, N. et al. (1998) Cell 92, 253-63.

2.  Guy, G.R. et al. (2009) J Endocrinol 203, 191-202.

3.  Minowada, G. et al. (1999) Development 126, 4465-75.

4.  Impagnatiello, M.A. et al. (2001) J Cell Biol 152, 1087-98.

5.  Hanafusa, H. et al. (2002) Nat Cell Biol 4, 850-8.

6.  Ozaki, K. et al. (2001) Biochem Biophys Res Commun 285, 1084-8.

7.  Thum, T. et al. (2008) Nature 456, 980-4.

8.  Edwin, F. et al. (2009) Mol Pharmacol 76, 679-91.


Entrez-Gene Id 10252
Swiss-Prot Acc. O43609


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.