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PhosphoPlus® Elk-1 (Ser383) Antibody Kit #9180
Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation by purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) Antibody (upper) or control Elk-1 Antibody #9182 (lower).Learn more about how we got this image
Western blot analysis of Elk-1 fusion protein phosphorylated in vitro with purified Erk2 enzyme, using control Elk-1 Antibody.Learn more about how we got this image
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.Learn more about how we got this image
Gallery: PhosphoPlus® Elk-1 (Ser383) Antibody Kit #9180
|Phospho-Elk-1 (Ser383) Antibody 9181||x 100 µl||
|Elk-1 Antibody 9182||x 100 µl||
||H M R Z||47||Rabbit|
|Elk-1 Control Proteins 9183||2 x 150 µl||
||40 kDa nonphosphorylated, 50 kDa phosphorylated||E. Coli|
|Anti-rabbit IgG, HRP-linked Antibody 7074||x 100 µl||
|Anti-biotin, HRP-linked Antibody 7075||x 100 µl||
|Biotinylated Protein Ladder Detection Pack 7727||x 100 µl||
|20X LumiGLO® Reagent and 20X Peroxide 7003||2 x 5 ml each||
The PhosphoPlus® Elk-1 (Ser383) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of Elk-1. Bacterially expressed Elk-1 serves as a negative control. The same protein phosphorylated by p42 MAP Kinase (Erk2) serves as a positive control.
Elk-1 Antibody detects endogenous levels of total Elk-1 protein. Phospho-Elk-1 (Ser383) Antibody detects EIk-1 protein only when activated by phosphorylation at serine 383.
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser383 of human Elk-1, or with a synthetic peptide derived from the sequence of human Elk-1. Antibodies are purified by protein A and peptide affinity chromatography.
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc. PhosphoPlus® is a trademark of Cell Signaling Technology, Inc. LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.