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9183S 150 µl (10 western blots) $109.00
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MW (kDa)
40 kDa nonphosphorylated, 50 kDa phosphorylated

Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation by purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) Antibody #9186 (upper) or control Elk-1 Antibody #9182 (lower).

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Product Description

Nonphosphorylated Elk1 fusion protein: Bacterially expressed Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer. Store at -20ºC.

Phosphorylated Elk1 fusion protein: Elk1 phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.


Product Usage Information

As controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.


Storage: Store at -20°C.

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).


Western Blots: For controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.


1.  Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.

2.  Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.

3.  Whitmarsh, A.J. et al. (1995) Science 269, 403-407.

4.  Marais, R. et al. (1993) Cell 73, 381-93.

5.  Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.


Entrez-Gene Id 2002
Swiss-Prot Acc. P19419


For Research Use Only. Not For Use In Diagnostic Procedures.
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