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23 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

RGS4 Antibody recognizes endogenous levels of total RGS4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys33 of human RGS4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Regulators of G protein signaling (RGS) are a family of about 40 proteins that determine the signaling amplitude and duration of G protein-coupled receptor signaling via modulation of the GTPase activity of G proteins (1-3). Each RGS has a distinct structure, expression pattern, and regulation, resulting in preferential interactions with receptors, G proteins, and other signaling proteins, as well as a unique function (4).

RGS4 is a small RGS of 23 kDa that belongs to the R4 subfamily of RGS. It harbors a short N-terminus thought to be critical for its functional specificity towards GPCRs and a RGS homology domain (RH domain) (5). RGS4 shares activity at both Gαq (6) and Gαi/o proteins (7).

RGS4 degradation is regulated by opioid receptor-mediated ubiquitination after stimulation by opioid receptor agonists such as DAMGO (8); and RGS4 modulates the action of morphine and other opiates in vivo (9).

1.  Hollinger, S. and Hepler, J.R. (2002) Pharmacol Rev 54, 527-59.

2.  Traynor, J.R. and Neubig, R.R. (2005) Mol Interv 5, 30-41.

3.  Neubig, R.R. and Siderovski, D.P. (2002) Nat Rev Drug Discov 1, 187-97.

4.  Terzi, D. et al. (2009) Prog Mol Biol Transl Sci 86, 299-333.

5.  Hollinger, S. and Hepler, J.R. (2002) Pharmacol Rev 54, 527-59.

6.  Xu, X. et al. (1999) J Biol Chem 274, 3549-56.

7.  Berman, D.M. et al. (1996) Cell 86, 445-52.

8.  Wang, Q. and Traynor, J.R. (2011) J Biol Chem 286, 7854-64.

9.  Han, M.H. et al. (2010) Biol Psychiatry 67, 761-9.

Entrez-Gene Id 5999
Swiss-Prot Acc. P49798

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

RGS4 Antibody