Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

REACTIVITY SENSITIVITY MW (kDa) SOURCE
23 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

RGS4 Antibody recognizes endogenous levels of total RGS4 protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys33 of human RGS4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Regulators of G protein signaling (RGS) are a family of about 40 proteins that determine the signaling amplitude and duration of G protein-coupled receptor signaling via modulation of the GTPase activity of G proteins (1-3). Each RGS has a distinct structure, expression pattern, and regulation, resulting in preferential interactions with receptors, G proteins, and other signaling proteins, as well as a unique function (4).


RGS4 is a small RGS of 23 kDa that belongs to the R4 subfamily of RGS. It harbors a short N-terminus thought to be critical for its functional specificity towards GPCRs and a RGS homology domain (RH domain) (5). RGS4 shares activity at both Gαq (6) and Gαi/o proteins (7).

RGS4 degradation is regulated by opioid receptor-mediated ubiquitination after stimulation by opioid receptor agonists such as DAMGO (8); and RGS4 modulates the action of morphine and other opiates in vivo (9).


1.  Hollinger, S. and Hepler, J.R. (2002) Pharmacol Rev 54, 527-59.

2.  Traynor, J.R. and Neubig, R.R. (2005) Mol Interv 5, 30-41.

3.  Neubig, R.R. and Siderovski, D.P. (2002) Nat Rev Drug Discov 1, 187-97.

4.  Terzi, D. et al. (2009) Prog Mol Biol Transl Sci 86, 299-333.

5.  Hollinger, S. and Hepler, J.R. (2002) Pharmacol Rev 54, 527-59.

6.  Xu, X. et al. (1999) J Biol Chem 274, 3549-56.

7.  Berman, D.M. et al. (1996) Cell 86, 445-52.

8.  Wang, Q. and Traynor, J.R. (2011) J Biol Chem 286, 7854-64.

9.  Han, M.H. et al. (2010) Biol Psychiatry 67, 761-9.


Entrez-Gene Id 5999
Swiss-Prot Acc. P49798


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.