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Western blot analysis of extracts from COS and 293 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb (upper) or p38 MAPK Antibody #9212 (lower).

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Western blot analysis of extracts from various cell lines using p38 MAPK (D13E1) XP® Rabbit mAb.

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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb.

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma using p38 MAPK (D13E1) XP® Rabbit mAb.

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Immunohistochemical analysis of paraffin-embedded 293T cell pellets, untreated (left) or UV-treated (right), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb.

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Immunohistochemical analysis of paraffin-embedded human colon carcinoma using p38 MAPK (D13E1) XP® Rabbit mAb.

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Flow cytometric analysis of Jurkat cells, untreated (blue) or anisomycin-treated (green), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb compared to a nonspecific negative control antibody (red).

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Immunohistochemical analysis of paraffin-embedded human lung carcinoma using p38 MAPK (D13E1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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Confocal immunofluorescent analysis of COS cells, untreated (left) or anisomycin-treated (right) using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

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Flow cytometric analysis of HeLa cells using p38 MAPK (D13E1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

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Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with UV (100 mJ/cm2 with 30 min recovery; right), using p38 MAPK (D13E1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

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Image
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb 4511 x 200 µl
H M R Mk Mi Pg Sc 43 Rabbit IgG
p38 MAPK (D13E1) XP® Rabbit mAb 8690 x 100 µl
H M R Hm Mk Pg 40 Rabbit IgG
p38 MAPK Control Cell Extracts 9213 x 150 µl
 
Anti-rabbit IgG, HRP-linked Antibody 7074 x 100 µl
All Goat 
Anti-biotin, HRP-linked Antibody 7075 x 250 µl
Goat 
Biotinylated Protein Ladder Detection Pack 7727 x 250 µl
 
20X LumiGLO® Reagent and 20X Peroxide 7003 x 10 ml
 

Product Description

The PhosphoPlus® p38 MAPK (Thr180/Tyr182) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of p38 MAPK at Thr180/Tyr182.


Specificity / Sensitivity

Phospho-p38 MAPK Antibody detects phosphorylated threonine 180 and tyrosine 182 of p38 MAPK but does not appreciably cross-react with the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK. p38 MAPK (D13E1) XP® Rabbit mAb recognizes endogenous levels of total p38α, ß, or γ MAPK protein and does not recognize p38δ, SAPK/JNK, or p44/42 MAPK proteins.


Source / Purification

Monoclonal activation state antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p38 protein.

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).

SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).


1.  Han, J. et al. (1994) Science 265, 808-11.

2.  Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.

3.  Rouse, J. et al. (1994) Cell 78, 1027-1037.

4.  Lee, J.C. et al. (1994) Nature 372, 739-46.

5.  Freshney, N.W. et al. (1994) Cell 78, 1039-49.

6.  Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.

7.  Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.

8.  Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.

9.  Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.

10.  Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.