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90 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-RSK2 (Tyr529) Antibody detects endogenous RSK2 protein only when phosphorylated at Tyr529. This antibody cross-reacts with some tyrosine phosphorylated proteins including EGFR.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human RSK2. Antibodies are purified by protein A and peptide affinity chromatography.

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

Phosphorylation of RSK2 on Tyr529 was identified at Cell Signaling Technology (CST) using PhosphoScan®, our LC-MS/MS platform for phosphorylation site discovery. This study identifies RSK2 as a key substrate of FGFR3 in human t(4;14)-positive multiple myeloma (MM) cells. Constitutively active FGFR3 directly phosphorylates RSK2 on Tyr529, which primes RSK2 for activation by ERK1/2 (4). RSK2 can also be phosphorylated at Tyr529 by Src family tyrosine kinases Src and Fyn in response to EGF stimulation (5). Mutations in the RSK2 gene are associated with Coffin-Lowry syndrome (CLS), an X-linked disorder characterized by mental retardation and the presence of characteristic facial anomalies (6).

1.  Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.

2.  Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.

3.  Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

4.  Kang, S. et al. (2007) Cancer Cell 12, 201-14.

5.  Kang, S. et al. (2007) J Biol Chem Submitted for Publication

6.  Delaunoy, J.P. et al. (2006) Clin Genet 70, 161-6.

Entrez-Gene Id 6197
Swiss-Prot Acc. P51812

For Research Use Only. Not For Use In Diagnostic Procedures.
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