Upstream / Downstream
Explore pathways related to this product.
CST Antibody Performance Guarantee
Find answers on our FAQs page.
PTM information and tools available.
Phospho-RSK2 (Tyr529) Antibody #9324
This product is discontinued
Gallery: Phospho-RSK2 (Tyr529) Antibody #9324
Phospho-RSK2 (Tyr529) Antibody detects endogenous RSK2 protein only when phosphorylated at Tyr529. This antibody cross-reacts with some tyrosine phosphorylated proteins including EGFR.
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human RSK2. Antibodies are purified by protein A and peptide affinity chromatography.
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).
Phosphorylation of RSK2 on Tyr529 was identified at Cell Signaling Technology (CST) using PhosphoScan®, our LC-MS/MS platform for phosphorylation site discovery. This study identifies RSK2 as a key substrate of FGFR3 in human t(4;14)-positive multiple myeloma (MM) cells. Constitutively active FGFR3 directly phosphorylates RSK2 on Tyr529, which primes RSK2 for activation by ERK1/2 (4). RSK2 can also be phosphorylated at Tyr529 by Src family tyrosine kinases Src and Fyn in response to EGF stimulation (5). Mutations in the RSK2 gene are associated with Coffin-Lowry syndrome (CLS), an X-linked disorder characterized by mental retardation and the presence of characteristic facial anomalies (6).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.