Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb 3073 x 40 µl
H 145 Rabbit IgG
Phospho-c-Kit (Tyr719) Antibody 3391 x 40 µl
H M 120, 145 Rabbit 
c-Kit (D13A2) XP® Rabbit mAb 3074 x 40 µl
H M 120 and 145 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 x 100 µl
All Goat 

Product Description

The c-Kit Antibody Sampler Kit provides a fast and economical means of evaluating levels of c-Kit receptor protein phosphorylated at the specified sites, as well as total c-Kit receptor levels. The kit contains enough primary and secondary antibody to perform four Western blot experiments.


Specificity / Sensitivity

Phospho-c-Kit antibodies detect endogenous levels of c-Kit when phosphorylated at the specified sites. c-Kit (D13A2) XP® Rabbit mAb detects endogenous levels of total c-kit receptor protein.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Tyr703 of human c-Kit. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr719 of mouse c-Kit. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).


1.  Martin, F.H. et al. (1990) Cell 63, 203-11.

2.  Yarden, Y. et al. (1987) EMBO J 6, 3341-51.

3.  Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.

4.  Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.

5.  Nocka, K. et al. (1990) EMBO J 9, 1805-13.

6.  Hirota, S. et al. (1998) Science 279, 577-80.

7.  Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.


Entrez-Gene Id 3815
Swiss-Prot Acc. P10721


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.