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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Zap-70 (D1C10E) XP® Rabbit mAb 3165 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M 70 Rabbit IgG
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb 2717 40 µl
Western Blotting Immunofluorescence Flow Cytometry
H 70, 72 Rabbit IgG
SLP-76 Antibody 4958 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M 76 Rabbit 
Phospho-LAT (Tyr191) Antibody 3584 40 µl
Western Blotting Immunoprecipitation
H 36, 38 Rabbit 
LAT Antibody 9166 40 µl
Western Blotting Immunohistochemistry Flow Cytometry
H M 36, 38 Rabbit 
Phospho-Lck (Tyr505) Antibody 2751 40 µl
Western Blotting Immunoprecipitation
H M 56 Rabbit 
Lck (73A5) Rabbit mAb 2787 40 µl
Western Blotting Immunofluorescence Flow Cytometry
H 56 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The T Cell Signaling Antibody Sampler Kit contains primary and secondary antibodies to perform four Western blots with each antibody.

Specificity / Sensitivity

Each antibody in the T Cell Signaling Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members with the following exceptions: Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb which also recognizes Syk when phosphorylated at Tyr352; and Phospho-Lck (Tyr505) Antibody which may cross-react with certain phosphorylated Src family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human Zap-70; Tyr319 of human Zap-70; and Tyr505 of human Lck.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human SLP-76; residues surrounding Tyr191 of human LAT; the central region of human LAT; and residues surrounding Tyr505 of human Lck. Antibodies are purified by protein A and peptide affinity chromatography.

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (9). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (10,11). The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (11-12).

1.  Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.

2.  Iwashima, M. et al. (1994) Science 263, 1136-9.

3.  Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.

4.  Chan, A.C. et al. (1995) EMBO J 14, 2499-508.

5.  Williams, B.L. et al. (1999) EMBO J 18, 1832-44.

6.  Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.

7.  Wiestner, A. et al. (2003) Blood 101, 4944-51.

8.  Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.

9.  Zhang, W. et al. (1998) Cell 92, 83-92.

10.  Paz, P.E. et al. (2001) Biochem J 356, 461-71.

11.  Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83.

12.  Zhang, W. et al. (2000) J Biol Chem 275, 23355-61.

Entrez-Gene Id 27040 , 3932 , 3937 , 6850 , 7535
Swiss-Prot Acc. O43561 , P06239 , Q13094 , P43405 , P43403

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.