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62 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Smad6 Antibody detects endogenous levels of total Smad6 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding cysteine 55 of human Smad6. Antibodies were purified by protein A and peptide affinity chromatography.

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

The inhibitory Smads, Smad6 and Smad7, were originally identified in vascular endothelium and inhibit TGF-β and BMP signaling by interfering with the phosphorylation of other Smad family members (9,10). Their expression may be induced by TGF-β, and BMP in some cell types providing a negative feedback loop (11,12).

1.  Heldin, C.H. et al. (1997) Nature 390, 465-71.

2.  Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.

3.  Derynck, R. et al. (1998) Cell 95, 737-40.

4.  Massagué, J. (1998) Annu Rev Biochem 67, 753-91.

5.  Whitman, M. (1998) Genes Dev 12, 2445-62.

6.  Wu, G. et al. (2000) Science 287, 92-7.

7.  Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.

8.  Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.

9.  Topper, J.N. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 9314-9319.

10.  Imamura, T. et al. (1997) Nature 389, 622-626.

11.  Takase, M. et al. (1998) Biochem. Biophys. Res. Commun. 244, 26-29.

12.  Afrakhte, M. et al. (1998) Biochem. Biophys. Res. Commun. 249, 505-511.

Entrez-Gene Id 4091
Swiss-Prot Acc. O43541

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Smad6 Antibody