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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Slug (L40C6) Mouse mAb detects endogenous levels of total Slug protein. It does not recognize transfected human or mouse Snail proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human Slug protein.

Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5). The tumor suppressor protein p53 induces Slug expression in γ-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7).

1.  Castro Alves, C. et al. (2007) J Pathol 211, 507-15.

2.  Wu, W.S. et al. (2005) Cell 123, 641-53.

3.  Pérez-Mancera, P.A. et al. (2006) Cytogenet Genome Res 114, 24-9.

4.  Inukai, T. et al. (1999) Mol Cell 4, 343-52.

5.  Bolós, V. et al. (2003) J Cell Sci 116, 499-511.

6.  Turner, F.E. et al. (2006) J Biol Chem 281, 21321-31.

7.  Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-61.

Entrez-Gene Id 6591
Swiss-Prot Acc. O43623

Protein Specific References

Kim JY et al. (2012) FEBS J 279, 2929–39

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Slug (L40C6) Mouse mAb