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Leptomycin B #9676
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with LMB (200 nM, 18 hr ), using p53 (7F5) Rabbit mAb #2527 and β-Actin (13E5) Rabbit mAb #4970.Learn more about how we got this image
Confocal immunofluorescent analysis of IGROV-1 cells, treated with LY294002 #9901 (50 μM, 3hr; left), insulin (100 ng/mL, 30 min; middle), or Leptomycin B followed by insulin (20 nM, 3hr and 100 ng/mL, 30 min, respectively; right), using FoxO1 (C29H4) Rabbit mAb #2880 (green). Actin filaments were labeled with DY-554 phalloidin (red).Learn more about how we got this image
Gallery: Leptomycin B #9676
Leptomycin B is supplied as a 200 μM solution in ethanol within a sealed container. Please use a needle and syringe to remove the solution from the vial. All dilutions, except the final dilution, must be performed in ethanol. Final dilutions can be performed in culture media. Working concentrations and length of treatment can vary depending on the desired effect, but 1-20 nM for 3 hours generally inhibits most nuclear export. Soluble and stable in ethanol. Leptomycin B is not stable in DMSO; do not dilute in DMSO.
In order to minimize evaporation, it is recommended that the LMB vial be kept on ice when in use.
Stability Warning: LMB in any quantity is unstable when dried down into a film. Thus, under no circumstances should the solvent be removed from solutions of LMB because rapid decomposition and loss of recoverable material will result.Storage: Store lyophilized or in solution at -20°C. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency.
Leptomycin B (LMB), originally discovered and utilized as a potent anti-fungal antibiotic from Streptomyces sp., has more recently been identified to inhibit nuclear export of proteins and RNA containing a Nuclear Export Sequence (NES) (1). The mechanism behind LMB's potent inhibition is achieved by specifically binding to chromosomal region maintenance (CRM)/exportin 1 protein; CRM1 binds to ribonuclear proteins containing the NES (1,2). LMB has also been reported to inhibit the degredation and subsequently lead to accumulation of p53 within the nucleus (3) and has demontrated specific anti-tumor properties, although toxic, at high doses (1-3).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.