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63 to 66 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Caspase-10 Antibody detects endogenous levels of full length caspase-10 and its various isoforms. The antibody does not cross-react with other caspases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding aspartic acid 219 of human caspase-10. Antibodies are purified by protein A and peptide affinity chromatography.

Caspase-10 is a DED (death effector domain)-containing caspase and functions as an initiator caspase in Fas/TNF induced apoptosis (1). Four isoforms of caspase-10 have been identified: caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d. They have the same prodomain but different mature large and small subdomains (2-4). Upon death ligand-receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction (1-4). This complex processes procaspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, leading to apoptosis (3-6).

1.  Nunez, G. et al. (1998) Oncogene 17, 3237-3245.

2.  Ng, P. W. et al. (1999) J. Biol. Chem. 274, 10301-10308.

3.  Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

4.  Srinivasula, S. M. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 14486-14491.

5.  Wang, J. et al. (1999) Cell 98, 47-58.

6.  Vincenz, C. and Dixit, V.M. (1997) J Biol Chem 272, 6578-83.

Entrez-Gene Id 843
Swiss-Prot Acc. Q92851

For Research Use Only. Not For Use In Diagnostic Procedures.
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