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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
β-Actin (8H10D10) Mouse mAb 3700 1 x 40 µl
H M R Hm Mk Dg 45 Mouse IgG2b
α-Tubulin (DM1A) Mouse mAb 3873 1 x 40 µl
H M R Mk 52 Mouse IgG1
Histone H3 (96C10) Mouse mAb 3638 1 x 40 µl
H M R Mk 17 Mouse IgG1
Anti-mouse IgG, HRP-linked Antibody 7076 1 x 100 µl
All Horse 

Product Description

The Loading Control Antibody Sampler Kit provides an economical means to detect a variety of housekeeping proteins. The kit contains enough primary and secondary antibodies to perform four western blot experiments.


Specificity / Sensitivity

Each antibody in the Loading Control Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross-react with other proteins.


Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino terminus of human β-actin, the carboxy terminus of human histone H3, or with full-length chicken α-tubulin purfied from brain extracts. The epitope of α-Tubulin (DM1A) Mouse mAb recognizes residues surrounding Val440 of human α-tubulin protein.

Housekeeping proteins perform numerous basic functions within the cell and are constitutively expressed at high levels in a variety of tissues and cell types. Western blot analysis commonly uses housekeeping proteins such as β-actin, histone H3, and α-tubulin as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Actin exists mainly as the F-actin fibrous polymer (1). Globular tubulin subunits made up of α- and β-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton (2). Histone proteins, including histone H3, make up the primary building block of chromatin known as nucleosomes. Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes (3).


1.  Westermann, S. and Weber, K. (2003) Nat. Rev. Mol. Cell Biol. 4, 938 -947.

2.  Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.

3.  Condeelis, J. (2001) Trends Cell Biol 11, 288-93.


Entrez-Gene Id 60, 8350, 10376
Swiss-Prot Acc. P60709, P68431, P68363


For Research Use Only. Not For Use In Diagnostic Procedures.
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