Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Neurofilament-M (RMO 14.9) Mouse mAb 2838 x 40 µl
H M R 160 Mouse IgG1
Neurofilament-L (DA2) Mouse mAb 2835 x 40 µl
H M R 70 Mouse IgG1
Neurofilament-L (C28E10) Rabbit mAb 2837 x 40 µl
H M R 70 Rabbit IgG
Neurofilament-H (RMdO 20) Mouse mAb 2836 x 40 µl
H M R 180-220 Mouse IgG1
Anti-rabbit IgG, HRP-linked Antibody 7074 x 100 µl
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 x 100 µl
All Horse 

Product Description

The Neurofilament Antibody Sampler Kit provides an economical means of evaluating neurofilaments. The kit contains enough primary and secondary antibodies to perform four western blot experiments per primary antibody.


Specificity / Sensitivity

Neurofilament-L (DA2) Mouse mAb and Neurofilament-L (C28E10) Rabbit mAb detect endogenous levels of total Neurofilament-L protein. Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein. Neurofilament-H (RMdO 20) Mouse mAb detects endogenous levels of total Neurofilament-H protein. Neurofilament-H (RMdO 20) Mouse mAb has been reported to detect NFM and NFH in human samples but only NFH in mouse, rat or bovine samples (Lee, V.M. et al., 1988).


Source / Purification

Monoclonal antibodies are produced by immunizing animals with purified and enzymatically dephosphorylated pig neurofilament, light chain (Neurofilament-L (DA2)), a synthetic peptide surrounding Glu450 of human Neurofilament-L (Neurofilament-L (C28E10)), rat neurofilament medium chain

(Neurofilament-M (RMO 14.9)), or rat neurofilament (Neurofilament-H (RMdO 20)).

#2836 Monoclonal antibody is produced by immunizing animals with rat neurofilament.

#2835 Monoclonal antibody is produced by immunizing animals with purified and enzymatically dephosphorylated pig neurofilament, light chain.

#2837 Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L.

#2838 Monoclonal antibody is produced by immunizing animals with rat neurofilament, medium chain.

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).


1.  Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.

2.  Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.

3.  Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

4.  Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55.


Entrez-Gene Id 4744, 4741, 4747
Swiss-Prot Acc. P12036, P07197, P07196


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.