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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
p38α MAPK Antibody 9218 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Mk 40 Rabbit 
p38β MAPK (C28C2) Rabbit mAb 2339 40 µl
Western Blotting Immunoprecipitation
H Mk 43 Rabbit IgG
p38γ MAPK Antibody 2307 40 µl
Western Blotting Immunoprecipitation
H M R Mk 46 Rabbit 
p38δ MAPK (10A8) Rabbit mAb 2308 40 µl
Western Blotting Immunoprecipitation
H R 43 Rabbit IgG
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb 4511 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk Mi Pg Sc 43 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The p38 MAPK Isoform Activation Antibody Sampler Kit provides an economical means to evaluate the activation status of individual isoforms of p38 MAPK through immunoprecipitation of the phosphorylated p38 MAPK followed by western blot using isoform specific antibodies. The kit includes enough primary and secondary antibodies to perform four IP/western blot experiments.


Specificity / Sensitivity

Each isoform-specific antibody in the p38 MAPK Isoform Activation Antibody Sampler Kit recognizes the appropriate isoform of p38 MAPK. The Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb recognizes all four isoforms of p38 MAPK when phosphorylated at the specified sites.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human p38α MAPK or human p38γ MAPK. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of p38β MAPK or p38δ MAPK and surrounding Thr180/Tyr182 of human p38 MAPK.

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).

SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).


1.  Rouse, J. et al. (1994) Cell 78, 1027-37.

2.  Han, J. et al. (1994) Science 265, 808-11.

3.  Lee, J.C. et al. (1994) Nature 372, 739-46.

4.  Freshney, N.W. et al. (1994) Cell 78, 1039-49.

5.  Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.

6.  Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.

7.  Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30.

8.  Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38.

9.  Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.

10.  Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.


Entrez-Gene Id 1432 , 5600 , 5603 , 6300
Swiss-Prot Acc. Q16539 , Q15759 , O15264 , P53778


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.