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RIPA Buffer (10X) #9806
Gallery: RIPA Buffer (10X) #9806
RIPA buffer is used to lyse cells and tissues.
1. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For longer periods of time, buffer should be stored at –20°C. Aliquotting of 10X buffer is recommended if many small experiments are to be performed.
2. Thaw 10x buffer at 24-30°C, mixing end-over-end.
3. Dilute 10X RIPA Buffer to a 1X solution using ddH2O. This product supplies enough 10X material to make 150mls of whole cell extract.
4. Chill 1X buffer on ice and add PMSF just prior to use.Solutions and Reagents:
20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3VO4, 1 µg/ml leupeptin.
Note: CST recommends adding 1 mM PMSF immediately before use.Storage: Store at -20°C
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.