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9870S 1 Kit (8 x 40 µl) $519.00
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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb 4539 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M R Mk 34 Rabbit IgG
Cyclin A2 (BF683) Mouse mAb 4656 40 µl
Western Blotting Flow Cytometry
H 55 Mouse IgE
Cyclin B1 (D5C10) XP® Rabbit mAb 12231 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H R 55 Rabbit IgG
Cyclin E2 Antibody 4132 40 µl
Western Blotting
H 48 Rabbit 
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb 3377 40 µl
Western Blotting Immunofluorescence Flow Cytometry
H M R Mk Z 17 Rabbit IgG
Myt1 Antibody 4282 40 µl
Western Blotting Immunohistochemistry
H M R 60 to 70 Rabbit 
p21 Waf1/Cip1 (12D1) Rabbit mAb 2947 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H Mk 21 Rabbit IgG
Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb 4910 40 µl
Western Blotting Immunoprecipitation
H 95 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
All Horse 

Product Description

The Cell Cycle Regulation Sampler Kit II provides an economical means of evaluating cell cycle proteins. The kit contains enough primary and secondary antibodies to perform four western blot experiments.

Specificity / Sensitivity

Each antibody in the Cell Cycle Regulation Sampler Kit II detects endogenous levels of its target protein and does not typically cross react with other family members. Cyclin B1 (D5C10) XP® Rabbit mAb recognizes endogenous levels of total cyclin B1 protein. This antibody also detects a 100 kDa protein of unknown origin in some cell lines. Activation state antibodies recognize target proteins only when phosphorylated at the indicated residue. Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb detects endogenous levels of cdc2 protein only when phosphorylated at tyrosine 15. Based on sequence similarity, the antibody may cross-react with CDK2 and CDK3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy-terminus of human cyclin E2 or the middle of mouse and human Myt1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy-terminus of human p21, residues near the amino terminus of human cyclin B1 protein, or recombinant human cyclin A2 protein. Activation state monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser10 of human histone H3, residues surrounding Ser642 of human Wee1, or residues surrounding Tyr15 of human cdc2.

The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Tyr15 and Thr14 (1). Phosphorylation of cdc2 by the protein kinases Wee1 and Myt1 at Thr14 and Tyr15 results in inhibition of cdc2 (2,3). Progression through the G1/S checkpoint and initiation of DNA replication requires cyclin E; traversing the G2/M checkpoint to initiate mitosis requires cyclin B, and cyclin A may be required for both S-phase and M-phase (4). The versatile p21 cyclin-dependent kinase inhibitor, which interacts with several cyclin-CDK complexes to regulate cyclin-CDK during the cell cycle, is regulated by phosphorylation and ubiquitin-mediated degradation (5). Phosphorylation of histone H3 at Ser10 and neighboring residues correlates with chromosomal condensation, which is essential for segregation of chromosomes during mitosis (6).

1.  Norbury, C. et al. (1991) EMBO J 10, 3321-9.

2.  McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.

3.  Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.

4.  Pagano, M. et al. (1992) EMBO J 11, 961-71.

5.  Abbas, T. and Dutta, A. (2009) Nat Rev Cancer 9, 400-14.

6.  Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.

Entrez-Gene Id 5901 , 7465
Swiss-Prot Acc. P62826 , P30291

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.