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TPA (Phorbol-12-Myristate-13-Acetate) #9905
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Gallery: TPA (Phorbol-12-Myristate-13-Acetate) #9905
TPA is supplied as a 200 μM stock in DMSO. For standard 200 nM treatment, dilute 1000X.Storage: Store lyophilized or in solution at -20°C. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency.
TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is soluble in DMSO and ethanol. DMSO solutions can be stored at -20ºC, in the absence of light, for at least six months. A 200 nM TPA treatment is standard to induce signaling.
TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.