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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Stat1 Antibody 9172 1 x 40 µl
H M R Mk 84, 91 Rabbit 
Stat3 (79D7) Rabbit mAb 4904 1 x 40 µl
H M R Mk 79, 86 Rabbit IgG
Stat5 (3H7) Rabbit mAb 9358 1 x 40 µl
H M R 90 Rabbit IgG
Stat6 (D3H4) Rabbit mAb 5397 1 x 40 µl
H M R 110 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 1 x 100 µl
All Goat 

Product Description

The Stat Antibody Sampler Kit provides an economical means to examine multiple Stat proteins: Stat1, Stat3, Stat5 and Stat6. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.


Specificity / Sensitivity

Each Stat antibody in the kit recognizes only its target protein, independent of phosphorylation state.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human Stat1. Antibodies are purified by protein A and peptide affinity chromatography. Stat5 (3H7) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the central region of human Stat5. Stat6 (D3H4) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly582 of human Stat6 protein. Stat3 (79D7) Rabbit mAb is produced by immunizing animals with GST-Stat3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3 protein.

Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).


1.  Darnell, J.E. et al. (1994) Science 264, 1415-1421.

2.  Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.

3.  Caldenhoven, E. et al. (1996) J Biol Chem 271, 13221-7.

4.  Bromberg, J.F. et al. (1999) Cell 98, 295-303.

5.  Lim, C.P. and Cao, X. (1999) J. Biol. Chem. 274, 31055-31061.

6.  Wen, Z. et al. (1995) Cell 82, 241-50.

7.  Su, L. et al. (1999) J. Biol. Chem. 274, 31770-31774.

8.  Yokogami, K. et al. (2000) Curr Biol 10, 47-50.

9.  Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.

10.  Cattaneo, E. et al. (1999) Trends Neurosci. 22, 365-369.

11.  Frank, D.A. (1999) Mol. Med. 5, 432-456.


Entrez-Gene Id 6772, 6774, 6776, 6777, 6778
Swiss-Prot Acc. P42224, P40763, P42229, P51692, P42226

Protein Specific References

Dimberg A et al. (2000) Blood 96, 2870–8

Mowen KA et al. (2001) Cell 104, 731–41

Zhu W et al. (2002) J Biol Chem 277, 35787–90

Chen Q et al. (2002) Immunology 107, 199–208

Nair JS et al. (2002) Proc Natl Acad Sci U S A 99, 5971–6

DeVries TA et al. (2004) J Biol Chem 279, 45603–12

Timofeeva OA et al. (2006) Oncogene 25, 7555–64

Liu X et al. (2008) J Immunol 181, 449–63

Vanhatupa S et al. (2008) Biochem J 409, 179–85

Roth A et al. (2010) Proc Natl Acad Sci U S A 107, 19502–7

Chen Z et al. (2011) Cell Signal 23, 1404–12

Antunes F et al. (2011) Mol Cell Biol 31, 3029–37

Grönholm J et al. (2012) BMC Biochem 13, 20

Ng J and Cantrell D (1997) J Biol Chem 272, 24542–9

Schuringa JJ et al. (2000) Blood 95, 3765–70

Duarte RF and Frank DA (2000) Blood 96, 3422–30

Schuringa JJ et al. (2001) Oncogene 20, 5350–8

Deb A et al. (2001) EMBO J 20, 2487–96

Abe K et al. (2001) Oncogene 20, 3464–74

Kopantzev Y et al. (2002) Oncogene 21, 6791–800

Sun S and Steinberg BM (2002) J Gen Virol 83, 1651–8

Hwang JH et al. (2003) Mol Endocrinol 17, 1155–66

Lo RK et al. (2003) J Biol Chem 278, 52154–65

Liu H et al. (2003) Blood 102, 344–52

Lo RK and Wong YH (2004) Mol Pharmacol 65, 1427–39

Wang R et al. (2005) J Biol Chem 280, 11528–34

Yuan ZL et al. (2005) Science 307, 269–73

Ray S et al. (2005) Gastroenterology 129, 1616–32

Liu AM et al. (2006) J Biol Chem 281, 35812–25

Zhang X et al. (2007) Proc Natl Acad Sci U S A 104, 4060–4

Ohbayashi N et al. (2007) Biol Pharm Bull 30, 1860–4

Nadiminty N et al. (2007) Biochem Biophys Res Commun 359, 379–84

Duechting A et al. (2008) J Virol 82, 7942–52

Chen CL et al. (2008) Mol Cancer 7, 78

Hatziapostolou M et al. (2011) Cell 147, 1233–47

Sestito R et al. (2011) FASEB J 25, 916–27

Gupta M et al. (2012) Leukemia 26, 1356–64

Fox EM et al. (2008) Mol Endocrinol 22, 1781–96

Chen H et al. (2011) Cell 147, 436–46

Shirakawa T et al. (2011) J Biol Chem 286, 4003–10


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U.S. Patent No. 5,675,063.