Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb 2078 x 40 µl
H M 85 (IKKalpha), 87 (IKKbeta) Rabbit 
Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb 2697 x 40 µl
H M R Mk 85 IKK-alpha 87 IKK-beta Rabbit IgG
Phospho-IKKα/β (Ser176/180) Antibody II 2694 x 40 µl
H M R Mk 85 IKK-alpha 87 IKK-beta Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 x 100 µl
All Goat 

Product Description

The Phospho-IKKalpha/beta (Ser176/180) Antibody Sampler Kit contains reagents to examine protein levels of IKKalpha when phosphorylated at Ser176/180 and IKKbeta when phosphorylated at Ser177/181. The kit contains primary and secondary antibodies to perform four Western blots with each antibody.


Specificity / Sensitivity

Phospho-IKKα/β (Ser176/180) Antibody, Phospho-IKKα/β (Ser176/180) Antibody II, and Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb detect IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181.


Source / Purification

Polyclonal antibodies #2687 and #2694 are produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα and Ser177/181 of IKKβ, respectively, and are purified by protein A and peptide affinity chromatography. Monoclonal antibody #2697 is produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα.

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).


1.  Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.

2.  Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.

3.  Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.

4.  Brown, K. et al. (1995) Science 267, 1485-8.

5.  Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.

6.  Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.

7.  Chen, Z.J. et al. (1996) Cell 84, 853-62.

8.  Zandi, E. et al. (1997) Cell 91, 243-52.

9.  Karin, M. (1999) Oncogene 18, 6867-74.

10.  DiDonato, J.A. et al. (1997) Nature 388, 548-54.

11.  Mercurio, F. et al. (1997) Science 278, 860-6.

12.  Johnson, L.N. et al. (1996) Cell 85, 149-58.

13.  Delhase, M. et al. (1999) Science 284, 309-13.


Entrez-Gene Id 1147, 3551
Swiss-Prot Acc. O15111, O14920


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.