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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
IKKα (3G12) Mouse mAb 11930 1 x 40 µl
H Mk 85 Mouse IgG1
IKKβ (D30C6) Rabbit mAb 8943 1 x 40 µl
H M R Mk 87 Rabbit IgG
IKKγ Antibody 2685 1 x 40 µl
H M R Mk 48 Rabbit 
IKKε Antibody 2690 1 x 40 µl
H M R 80 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 1 x 100 µl
All Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 x 100 µl
All Horse 

Product Description

The IKK Isoform Antibody Sampler Kit provides an economical means to investigate NFkappaB signaling within the cell. The kit contains primary and secondary antibodies to perform four Western blots with each antibody.


Specificity / Sensitivity

IKKα, IKKβ, IKKγ, and IKKε antibodies detect endogenous levels of total IKKα, IKKβ, IKKγ and IKKε proteins, respectively. These antibodies do not cross-react with IKK subunits other than their specified target.


Source / Purification

The IKKβ (D30C6) Rabbit mAb was produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IKKβ protein. The IKKα (3G12) Mouse mAb was produced by immunizing animals with a recombinant protein specific to a fragment of human IKKα protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of human, IKKε and IKKγ. Antibodies are purified by protein A and peptide affinity chromatography.

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).


1.  Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.

2.  Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.

3.  Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.

4.  Brown, K. et al. (1995) Science 267, 1485-8.

5.  Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.

6.  Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.

7.  Chen, Z.J. et al. (1996) Cell 84, 853-62.

8.  Zandi, E. et al. (1997) Cell 91, 243-52.

9.  Karin, M. (1999) Oncogene 18, 6867-74.

10.  DiDonato, J.A. et al. (1997) Nature 388, 548-54.

11.  Mercurio, F. et al. (1997) Science 278, 860-6.

12.  Johnson, L.N. et al. (1996) Cell 85, 149-58.

13.  Delhase, M. et al. (1999) Science 284, 309-13.


Entrez-Gene Id 1147, 3551, 9641, 8517
Swiss-Prot Acc. O15111, O14920, Q14164, Q9Y6K9


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.