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Compare Cell Signaling Technology's Erk antibodies against one another to determine optimal applications
We have several monoclonal antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IF-IC with human samples. These are as follows:
We have several monoclonal antibodies for p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IF-IC with human samples. These are p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 and p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696. For this application, p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 is preferred.
We have several monoclonal antibodies for studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated in IF-IC with mouse tissues. These are p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 and p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696. For this application, p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 is preferred.
We have several antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IHC-P with mouse samples. These are Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb #4376. These antibodies perform similarly for IHC-P with mouse samples, therefore, there is no preference for this application.
We have several monoclonal antibodies studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P for mouse samples. These are p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 and p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696. For this application, p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 is preferred, as p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696 could be problematic due to mouse-on-mouse (MOM) background staining.
We have several monoclonal antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IF-IC with mouse samples. These are as follows:
Exactly what is ELISA? Learn about the enzyme-linked immunosorbent assay, the specific interaction between antibody & antigen. Click here.
Phospho-Erk product lists, product citation lists, product selection tools, comparison tables and educational resources for MAPK signaling from Cell Signaling Technology.
We have several monoclonal antibodies for studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P with human samples. These are p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 and p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696. These antibodies perform similarly for IHC-P with human samples, therefore there is no preference for this application.
We have several antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IHC-P with human samples. These are Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb #4376. These antibodies perform similarly for IHC-P with human samples, therefore, there is no preference for this application.
Western blotting using fluorescent-conjugated secondary antibodies - accurate quantification and two-color multiplexing using primary antibodies from different species.
Case Study 3: Exceptional Reproducibility of XP Monoclonal Antibodies from Cell Signaling Technology.
Products and Related Resources for Cytokines and Inflammation SARS-CoV-2 Research
Products and Related Resources for Fibrosis SARS-CoV-2 Research
Catalog numbers in tabular format for control cell extracts and proteins with links to product pages.
Understand when to use immunohistochemistry (IHC) over any other technique including Immunocytochemistry, Western Blot and ELISA
The accuracy of western blot results relies heavily on the quality of the primary antibody used. CST™ antibodies are validated using multiple experiments.
Case Study 1: Exceptional Specificity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Overview of MAP Kinase signaling pathways, antibodies and related reagents, interactive pathway diagrams, and other technical resources.
Get the reagents and supplies for immunohistochemistry (IHC) protocols including antibodies, diluent, blocking buffer, chromogens, and controls
Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.
Cell Signaling Technology® products are prevalent in CiteAb's Top Antibodies of 2022 with 9 of the top 10 most cited primary antibodies from CST.
Western Blotting troubleshooting guide for easy to solve high and low background issues.
Immunohistochemistry is a method for studying antigen localization in tissue sections using antibodies. Learn sample preparation, labeling, & visualization.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.
A scientific resource for the PX protein domain containing information on structure, function, and domain binding to phospholipids.
We currently have 6 total p53 antibodies reactive to human p53. All of these antibodies except #2524 and #30313 were produced by using the full-length human p53 as the immunogen. We currently have 1 additional total p53 antibody that binds to rodent p53, p53 (D2H9O) Rabbit mAb #32532. Please see below the comments about the epitopes of specific p53 antibodies.The p53 (1C12) Mouse mAb #2524 was produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser20 of human p53.Our mapping experiments for the p53 (7F5) Rabbit mAb #2527 have found that the epitope lies within the first 50 amino acids of the protein.Past mapping experiments of the p53 Antibody #9282 lots 3 and 4 have found several p53 regions that showed reactivity to this antibody. The most reactive residues lie within the first 100 amino acids of human p53, however, some reactivity was seen with sequences that lie within the DNA binding domain (aa102-292, UniProt ID P04637-1). Later, addi…
