Cell Signaling Technology

Product Pathways - Glucose Metabolism

ATGL Blocking Peptide #1006

Description

This peptide is used to block ATGL (30A4) Rabbit mAb #2439 reactivity in immunohistochemistry protocols.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks ATGL (30A4) Rabbit mAb #2439 by immunohistochemistry.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brown fat using ATGL (30A4) Rabbit mAb #2439 in the presence of control peptide (left) or ATGL Blocking Peptide (right).

Applications

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

Background

Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6).

  1. Holm, C. et al. (1988) Science 241, 1503-1506.
  2. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
  3. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
  4. Zimmermann, R. et al. (2004) Science 306, 1383-1386.
  5. Villena, J.A. et al. (2004) J. Biol. Chem. 279, 47066-47075.
  6. Jenkins, C.M. et al. (2004) J. Biol. Chem. 279, 48968-48975.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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