Product Pathways - Apoptosis
Cleaved Caspase-8 (Asp391) Blocking Peptide #1011
Description
This peptide is used to block Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496 reactivity in immunohistochemistry protocols.
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496 by immunohistochemistry.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon using Cleaved Caspase-8 (Asp391) (18C8) RmAb #9496 in the presence of control peptide (left) or Cleaved Caspase-8 (Asp391) Blocking Peptide (right).
Applications
Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.
Directions for Use
For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.
Background
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
- Muzio, M. et al. (1996) Cell 85, 817-827.
- Boldin, M.P. et al. (1996) Cell 85, 803-815.
- Fernandes-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 7464-7469.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
