Product Pathways - Apoptosis / Autophagy
Cytochrome c Blocking Peptide #1033
Description
This peptide is used to block Cytochrome c (136F3) Rabbit mAb #4280 reactivity.
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Cytochrome c (136F3) Rabbit mAb #4280 by immunohistochemistry.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cytochrome c (136F3) Rabbit mAb (#4280) in the presence of control peptide (left) or Cytochrome c Blocking Peptide (right).
Applications
Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.
Directions for Use
For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.
Background
Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).
- Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
- Li, P. et al. (1997) Cell 91, 479-489 .
- Liu, X. et al. (1996) Cell 86, 147-157.
Application References
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Companion Products
- 4280 Cytochrome c (136F3) Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
