Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

COX IV Blocking Peptide #1034

Description

This peptide is used specifically to block COX IV Antibody #4844 and COX IV (3E11) Rabbit mAb #4850 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks COX IV Antibody #4844 and COX IV (3E11) Rabbit mAb #4850 by immunohistochemistry.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded colon carcinoma, using COX IV Antibody #4844 in the presence of control peptide (left) or COX IV Blocking Peptide (right).

Applications

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

Background

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.

  1. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
  2. Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
  3. Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
  4. Barrientos, A. et al. (2002) Gene 286, 53-63.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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