Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Mre11 Blocking Peptide #1035

Description

This peptide is used specifically to block Mre11 Antibody #4895 and Mre11 (31H4) Rabbit mAb #4847 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Mre11 Antibody #4895 and Mre11 (31H4) Rabbit mAb #4847 by immunohistochemistry.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Mre11 Antibody #4895 in the presence of control peptide (left) or Mre11 Blocking Peptide (right).

Applications

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

Background

Mre11, originally described in genetic screens from the yeast Saccharomyces cerevisiae in which mutants were defective in meiotic recombination (1), is a central part of a multisubunit nuclease composed of Mre11, Rad50 and Nbs1 (MRN) (2,3). The MRN complex plays a critical role in sensing, processing and repairing DNA double strand breaks. Defects lead to genomic instability, telomere shortening, aberrant meiosis and hypersensitivity to DNA damage (4). Hypomorphic mutations of Mre11 are found in ataxia-telangiectasia-like disease (ATLD), with phenotypes similar to mutations in ATM that cause ataxia-telangiectasia (A-T), including a predisposition to malignancy in humans (5). Cellular consequences of ATLD include chromosomal instability and defects in the intra-S phase and G2/M checkpoints in response to DNA damage. The MRN complex may directly activate the ATM checkpoint kinase at DNA breaks (6).

  1. Ajimura, M. et al. (1993) Genetics 133, 51-66.
  2. D'Amours, D. and Jackson, S.P. (2002) Nat. Rev. Mol. Cell Biol. 3, 317-327.
  3. van den Bosch, M. et al. (2003) EMBO Rep. 4, 844-849.
  4. Theuissen, J.F. et al. (2003) Mol. Cell 12, 1511-1523.
  5. Stewart, G.S. et al. (1999) Cell 99, 577-587.
  6. Carson, C.T. et al. (2003) EMBO J. 22, 6610-6620.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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