Product Pathways - MAPK Signaling
Phospho-MAPKAPK-2 (Thr334) Blocking Peptide #1041
Description
This peptide is used to block Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 reactivity. It can also be used to block Phospho-MAPKAPK-2 (Thr334) Antibody #3041.
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 signal in immunohistochemistry.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (#3007) in the presence of control peptide (left), or Phospho-MAPKAPK-2 (Thr334) Blocking Peptide (right).
Applications
Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.
Directions for Use
For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.
Background
In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).
Application References
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