Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Thioredoxin 1 Blocking Peptide #1057

Description

This peptide is used to block Thioredoxin 1 (C63C6) Rabbit mAb #2429 reactivity in immunohistochemistry protocols.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Thioredoxin 1 (C63C6) Rabbit mAb #2429 signal in immunohistochemistry.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue using Thioredoxin 1 (C63C6) Rabbit mAb #2429 in the presence of control peptide (left) or Thioredoxin 1 Blocking Peptide (right).

Applications

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.

Background

Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3) Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).

  1. Watson, W.H. et al. (2004) Toxicol. Sci. 78, 3-14.
  2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol. Sci. 26, 398-404.
  3. Saitoh, M. et al. (1998) EMBO J. 17, 2596-2606.
  4. Callister, M.E. et al. (2007) Intensive Care Med. 33, 364-367.
  5. Callister, M.E. et al. (2006) Thorax 61, 521-527.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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