Product Pathways - Glucose Metabolism
Acetyl-CoA Carboxylase Blocking Peptide #1062
Description
This peptide is used to block Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 or Acetyl-CoA Carboxylase Antibody #3662 reactivity by immunohistochemistry and Western blot.
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 and Acetyl-CoA Carboxylase Antibody #3662 by immunohistochemistry and Western blot.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 in the presence of control peptide (left) or Acetyl-CoA Carboxylase Blocking Peptide #1062 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Acetyl CoA Carboxylase Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
Applications
Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.
Directions for Use
For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.
Background
Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα is the predominant isoform found in liver, adipocytes and mammary gland, while the 280 kDa ACCβ is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).
- Ruderman, N.B. et al. (1999) Am. J. Physiol. 276, E1-E18.
- Ha, J. et al. (1994) J. Biol. Chem. 269, 22162-22168.
- Abu-Elheiga, L. et al. (2001) Science 291, 2613-2616.
- Levert, K.L. et al. (2002) J. Biol. Chem. 277, 16347-16350.
Application References
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