Product Pathways - Apoptosis / Autophagy

TRAF1 Blocking Peptide #1066

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Description

This peptide is used to block TRAF1 (45D3) Rabbit mAb #4715 reactivity in immunohistochemistry protocols.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks TRAF1 (45D3) Rabbit mAb #4715 signal in immunohistochemistry.

Applications

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.

Background

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation and stress responses.

1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
4. Rothe, M. et al. (1994) Cell 78, 681-692.

Application References

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Companion Products

• 4715 TRAF1 (45D3) Rabbit mAb

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.