Product Pathways - Translational Control
mTOR Blocking Peptide #1072
Description
This peptide is used to specifically block mTOR (7C10) Rabbit mAb #2983 reactivity.
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks mTOR (7C10) Rabbit mAb #2983 signal in immunohistochemistry.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using mTOR (7C10) Rabbit mAb (#2983) in the presence of control peptide (left) or mTOR Blocking Peptide (right).
Applications
Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.
Directions for Use
For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.
Background
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
No product specific background information is currently available for this product.
- Sabers, C.J. et al. (1995) J. Biol. Chem. 270, 815-822.
- Brown, E.J. et al. (1994) Nature 369, 756-758.
- Sabatini, D.M. et al. (1994) Cell 78, 35-43.
- Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
- Dennis, P.B. et al. (2001) Science 294, 1102-1105.
- Fang, Y. et al. (2001) Science 294, 1942-1945.
- Nave, B.T. et al. (1999) Biochem. J. 344 Pt 2, 427-431.
- Peterson, R.T. et al. (2000) J. Biol. Chem. 275, 7416-7423.
- Huang, S. and Houghton, P.J. (2003) Curr. Opin. Pharmacol. 3, 371-377.
Application References
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Companion Products
- 2983 mTOR (7C10) Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
