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BiP Blocking Peptide #1084

Description

This peptide is used to block BiP (C50B12) Rabbit mAb #3177 reactivity in immunohistochemistry and western blot protocols.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks BiP (C50B12) Rabbit mAb #3177 by immunohistochemistry.

Applications

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

Background

Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4).

1. Wabl, M. and Steinberg, C. (1982) Proc. Natl. Acad. Sci. USA 79, 6976-6978.
2. Haas, I.G. and Wabl, M. Nature 306, 387-389.
3. Munro, S. and Pelham, H.R. (1986) Cell 46, 291-300.
4. Kohno, K. et al. (1993) Mol. Cell Biol. 13, 877-890.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3177 BiP (C50B12) Rabbit mAb

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For Research Use Only. Not For Use In Diagnostic Procedures.