Cell Signaling Technology

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Phospho-Akt Substrates-1 Biotinylated Peptide #1087

Cell Signaling Technology offers a full line of protein kinases, substrates, antibody detection reagents and HTScan® kits. Browse our "Reagents for High-Throughput Screening" product listing or contact us at drugdiscovery@cellsignal.com.

Description

This biotinylated peptide contains an Akt substrate motif with a sequence of (RXRX(L/A)S*(R/F). The serine residue in the peptide has been chemically phosphorylated in the course of peptide synthesis. This phosphopeptide was generated for use as a positive control in kinase assays (Chk1 Kinase #7359, CaMK2α Kinase #7358), but it may also serve as a positive control in other heterogeneous or homogeneous kinase assays.

Quality Control

The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.

Peptide Core Sequence

RXRX(L/A)S*(R/F)

Molecular Weight

1466

Directions for Use

This phosphorylated peptide can be detected with the Phospho-(Ser/Thr) Akt Substrate Antibody #9611. Sample kinase assay protocol is attached.

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