Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - NF-kB Signaling

IRAK1 (D51G7) XP® Rabbit mAb (Biotinylated) #11123

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Mk Endogenous 78, 105 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IRAK1 (D51G7) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total IRAK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IRAK1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IRAK1 (D51G7) XP® Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for detection.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated IRAK1 (D51G7) XP® Rabbit mAb #4504.

Background

Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).

  1. Dinarello, C.A. (1996) Blood 87, 2095-147.
  2. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
  3. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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