Product Pathways - Metabolism
ACAT2 Antibody #11814
PhosphoSitePlus® protein, site, and accession data: ACAT2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 41 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 11814:
- Immunoprecipitation, Western Blotting
Specificity / Sensitivity
ACAT2 Antibody recognizes endogenous levels of total ACAT2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val167 of human ACAT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using ACAT2 Antibody.
Western Blotting
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human ACAT2 (hACAT2-DDK; +), using ACAT2 Antibody (upper), DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (middle), and GAPDH (14C10) Rabbit mAb #2118 (lower).
Background
Acetyl-CoA acetyltransferase 2 (ACAT2), also known as cytosolic acetoacetyl-CoA thiolase, plays a role in regulating lipid metabolism (1). It catalyzes the synthesis of acetoacetyl-CoA from two acetyl-CoA molecules, which is later converted into steroids (2).
Application References
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Companion Products
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
