Product Pathways - Lymphocyte Signaling
TCF12/HEB (D2C10) Rabbit mAb #11825
|W IP||H (Mk) (B) (Dg) (Hr)||Endogenous||85||Rabbit IgG|
Reactivity Key: H=Human Mk=Monkey B=Bovine Dg=Dog Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
TCF12/HEB (D2C10) Rabbit mAb recognizes endogenous levels of total TCF12/HEB protein. This antibody recognizes the TCF12/HEBCan isoform, but not the TCF12/HEBAlt isoform.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly115 of human TCF12 protein.
TCF12/HEB (T cell factor 12/HeLa E-box binding protein) is a member of the E proteins that are type I basic helix-loop-helix domain containing transcription factors that bind directly to the E-box DNA consensus sequence and control numerous developmental processes (1,2). E protein family members include E2A, E2-2, and TCF12/HEB. TCF12/HEB forms homo- or heterodimers with other E proteins to regulate transcription of target genes that play a critical role in the development of T cells, B cells, and plasmacytoid dendritic cells (2-4). The TCF12/HEB gene has two transcriptional start sites leading to the generation of the long form called HEBCan, and the short form, HEBAlt (5). The two isoforms are thought to have distinct functions with HEBCan playing a role throughout T cell development, while HEBAlt functions in the efficient generation of T cell precursors (5).
- Murre, C. (2005) Nat Immunol 6, 1079-86.
- Braunstein, M. and Anderson, M.K. (2012) Clin Dev Immunol 2012, 678705.
- Quong, M.W. et al. (2002) Annu Rev Immunol 20, 301-22.
- Kee, B.L. (2009) Nat Rev Immunol 9, 175-84.
- Wang, D. et al. (2006) J Immunol 177, 109-19.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7004 TMB Substrate
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 4865 E2A Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.