Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

TCF12/HEB (D2C10) Rabbit mAb #11825

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H (Mk) (B) (Dg) (Hr) Endogenous 85 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey  B=Bovine  Dg=Dog  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TCF12/HEB (D2C10) Rabbit mAb recognizes endogenous levels of total TCF12/HEB protein. This antibody recognizes the TCF12/HEBCan isoform, but not the TCF12/HEBAlt isoform.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly115 of human TCF12 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and MOLT-4 cells using TCF12/HEB (D2C10) Rabbit mAb.

Background

TCF12/HEB (T cell factor 12/HeLa E-box binding protein) is a member of the E proteins that are type I basic helix-loop-helix domain containing transcription factors that bind directly to the E-box DNA consensus sequence and control numerous developmental processes (1,2). E protein family members include E2A, E2-2, and TCF12/HEB. TCF12/HEB forms homo- or heterodimers with other E proteins to regulate transcription of target genes that play a critical role in the development of T cells, B cells, and plasmacytoid dendritic cells (2-4). The TCF12/HEB gene has two transcriptional start sites leading to the generation of the long form called HEBCan, and the short form, HEBAlt (5). The two isoforms are thought to have distinct functions with HEBCan playing a role throughout T cell development, while HEBAlt functions in the efficient generation of T cell precursors (5).

  1. Murre, C. (2005) Nat Immunol 6, 1079-86.
  2. Braunstein, M. and Anderson, M.K. (2012) Clin Dev Immunol 2012, 678705.
  3. Quong, M.W. et al. (2002) Annu Rev Immunol 20, 301-22.
  4. Kee, B.L. (2009) Nat Rev Immunol 9, 175-84.
  5. Wang, D. et al. (2006) J Immunol 177, 109-19.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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