Cell Signaling Technology

Product Pathways - Neuroscience

VAMP2 Antibody #11829

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) (B) Endogenous 13 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

VAMP2 antibody recognizes endogenous levels of total VAMP2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VAMP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, rat brain, and rat synaptosome using VAMP2 Antibody.

Background

Vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin) is part of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex (1). The SNARE complex is involved in vesicular transport and membrane fusion, a process regulated by calcium (2). In neurons, VAMP2 is predominantly inserted in presynaptic vesicle membranes. Assembly of VAMP2 with the plasma membrane SNAREs syntaxin 1 and SNAP25 is a key event necessary for membrane fusion and neurotransmitter release (2). In addition to this important function, VAMP2 is also involved in granule exocytosis in neutrophils (3) and release of bioactive peptides from cardiac myocytes (4) and juxtaglomerular cells (5).

  1. Baumert, M. et al. (1989) EMBO J 8, 379-84.
  2. Sudhof, T.C. (2004) Annu Rev Neurosci 27, 509-47.
  3. Mollinedo, F. et al. (2003) J Immunol 170, 1034-42.
  4. Ferlito, M. et al. (2010) J Mol Cell Cardiol 49, 791-800.
  5. Mendez, M. et al. (2011) J Biol Chem 286, 28608-18.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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