Cell Signaling Technology

Product Pathways - Development

WBP2 Antibody #11831

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 32 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

WBP2 Antibody recognizes endogenous levels of total WBP2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn239 of human WBP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using WBP2 Antibody.

Background

WW-domain binding protein 2 (WBP2) is an adaptor protein first identified in a screen for proteins that interact with YAP1 (1). WBP2 was subsequently shown to bind other WW domain-containing proteins, including TAZ and NEDD4-like ubiquitin-protein ligases (2-5). There is strong evidence for a conserved functional role for WBP2 in the Hippo kinase tumor suppressor pathway. WBP2 interaction is required for the oncogenic properties of TAZ in breast cancer cells (4) and the growth-promoting properties of the YAP homolog Yorkie in Drosophila (6). In vitro studies have also suggested that WBP2 may act as a transcriptional co-activator of both estrogen and progesterone receptors (7); this interaction was shown to be dependent upon tyrosine phosphorylation of WBP2 by c-Src and c-Yes kinases (8).

  1. Chen, H.I. and Sudol, M. (1995) Proc Natl Acad Sci U S A 92, 7819-23.
  2. Chen, H.I. et al. (1997) J Biol Chem 272, 17070-7.
  3. McDonald, C.B. et al. (2011) Biochemistry 50, 9616-27.
  4. Chan, S.W. et al. (2011) Oncogene 30, 600-10.
  5. Jolliffe, C.N. et al. (2000) Biochem J 351 Pt 3, 557-65.
  6. Zhang, X. et al. (2011) Cell Death Differ 18, 1346-55.
  7. Dhananjayan, S.C. et al. (2006) Mol Endocrinol 20, 2343-54.
  8. Lim, S.K. et al. (2011) FASEB J 25, 3004-18.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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