Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Tau (Ser202) Antibody #11834

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 50-80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Tau (Ser202) Antibody recognizes endogenous levels of Tau protein only when phosphorylated at Ser202.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser202 of human Tau protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from adult and neonatal mouse brain using Phospho-Tau (Ser202) Antibody. The phospho-specificity of the antibody was verified by blocking with a phospho or nonphosphopeptide.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Investigators have shown that Tau is phosphorylated during development and hyper-phosphorylated at Ser202 in Alzheimer's disease (4).

  1. Johnson , G.V. and Stoothoff , W.H. (2004) J. Cell Sci. 117, 5721-5729.
  2. Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476.
  3. Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.
  4. Goedert, M. et al. (1993) Proc Natl Acad Sci U S A 90, 5066-70.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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