Product Pathways - Antibody Sampler Kits
Cell Fractionation Antibody Sampler Kit #11843
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|MEK1/2 (D1A5) Rabbit mAb #8727||40 µl||W IF-IC F||H M R Mk (Hm) Dm (X) (Z) (B) (Dg) (Pg) (Ce)||46||Rabbit IgG|
|AIF (D39D2) XP® Rabbit mAb #5318||40 µl||W IP IF-IC||H M R Mk (B) (Dg)||67||Rabbit IgG|
|Histone H3 (D1H2) XP® Rabbit mAb #4499||40 µl||W IHC-P IF-IC||H M R Mk (Hm) (C) (Dm) (X) (Z) (B)||17||Rabbit IgG|
|Vimentin (D21H3) XP® Rabbit mAb #5741||40 µl||W IHC-P IF-IC F||H M R Mk||57||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Dm=D. melanogaster X=Xenopus Z=Zebrafish B=Bovine Dg=Dog Pg=Pig Ce=C. elegans
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the Cell Fractionation Antibody Sampler Kit recognizes endogenous levels of its respective target protein. The antibodies do not cross-react with other family members.Each antibody has been validated using the Cell Fractionation Kit #9038. Expression of these proteins may vary in different cells and tissues.
Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb #4499.
Western blot analysis of extracts from Jurkat, C2C12, and C6 cells using AIF (D39D2) XP® Rabbit mAb #5318.
Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb #5741.
The Cell Fractionation Antibody Sampler Kit provides an economical means for determining the purity of each distinctly separated subcellular fraction by western blot using Cell Signaling Technology's Cell Fractionation Kit #9038. This antibody sampler kit includes enough primary antibody to perform at least four western blots per primary antibody.
Source / Purification
Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, the carboxy terminus of human histone H3 protein, residues surrounding Ala220 of human MEK1 protein, and residues surrounding Arg45 of human vimentin protein.
Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. Antibodies in the Cell Fractionation Antibody Sampler Kit can be used as a marker to ensure that each subcellular fraction is efficiently separated from the next. MEK1 and MEK2, also called MAPK or Erk Kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade (1). Apoptosis-inducing factor (AIF) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (2). Core histone protein, H3 is one of the primary building blocks of chromatin that makes up the nucleosome (3). Vimentin is a cell-specific intermediate filament with mesenchyme origin that contributes to making up of the cytoskeleton (4). MEK1/2, AIF, histone H3, and vimentin localize to the cytoplasm, mitochondria, nucleus, and cytoskeleton, respectively.
- Crews, C.M. et al. (1992) Science 258, 478-80.
- Lipton, S.A. and Bossy-Wetzel, E. (2002) Cell 111, 147-50.
- Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
- Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
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* Product-specific protocol.
- 9038 Cell Fractionation Kit
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7722 Blue Loading Buffer Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
For Research Use Only. Not For Use In Diagnostic Procedures.