Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lineage Markers

LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #11845

Applications Reactivity Sensitivity MW (kDa) Isotype
IF-IC F H M (R) (Mk) Endogenous 28 Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total LIN28A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro201 of human LIN28A protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa (blue) and NTERA-2 cl.D1 (green) cells using LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cl.D1 (left) and HeLa (right) cells using LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 Phalloidin #8878 (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated LIN28A (D1A1A) XP® Rabbit mAb #8641.

Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

  1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
  2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
  3. Cho, W.C. (2007) Mol Cancer 6, 60.
  4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
  5. West, J.A. et al. (2009) Nature 460, 909-13.
  6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
  7. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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