HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) #11846
|11846S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|11846||carrier free and custom formulation / quantity||email request|
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|IP||1:20||Transfected Only||Rabbit IgG|
Applications Key: IP=Immunoprecipitation
Specificity / Sensitivity
HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) detects exogenously expressed proteins containing the HA epitope tag.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).
Immunoprecipitation of COS-7 cells transfected with a construct expressing HA-tagged Akt using Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) #8726 (Lane 2) or HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) (Lane 3). Lane 1 is 10% lysate. The western blot was probed using HA-Tag (6E2) Mouse mAb (HRP Conjugate) #2999.
This Cell Signaling Technology antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of HA-tagged recombinant proteins.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.