Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Transcription Factors

PAX5 (D19F8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #11852

Applications Reactivity Sensitivity Isotype
F H M (X) Endogenous Rabbit IgG

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  X=Xenopus
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PAX5 (D19F8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total PAX5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln350 of human PAX5 protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat (blue) and Ramos (green) cells using PAX5 (D19F8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PAX5 (D19F8) XP® Rabbit mAb #8970.

Background

Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).

  1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
  2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
  3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
  4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.

Application References

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Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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