Product Pathways - Translational Control
Upf2 (D3B10) Rabbit mAb #11875
PhosphoSitePlus® protein, site, and accession data: UPF2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 170 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 11875:
- Western Blotting
Specificity / Sensitivity
Upf2 (D3B10) Rabbit mAb recognizes endogenous levels of total Upf2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human Upf2 protein.
Western Blotting
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human Upf2 (hUpf2-Myc; +), using Upf2 (D3B10) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457.
Western Blotting
Western blot analysis of extracts from various cell lines using Upf2 (D3B10) Rabbit mAb.
Background
Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).
- Leeds, P. et al. (1991) Genes Dev 5, 2303-14.
- Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90.
- Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8.
- Isken, O. et al. (2008) Cell 133, 314-27.
- Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
